APS distinguishes between diagnosis and biosignatures.

Both concern evidence for life, but they operate differently.

Diagnosis evaluates biological organisation directly.
It typically involves:

  • perturbation,
  • organisational response,
  • endogenous restoration,
  • and assessment of viability-oriented regulation.

Diagnosis therefore tests whether a system actively sustains the conditions of its own persistence.

Biosignatures, by contrast, provide indirect evidence.
They are observational indicators that may suggest the presence of viability-oriented organisation when direct intervention is impossible.

Examples include:

  • atmospheric disequilibrium,
  • persistence far from equilibrium,
  • regulated energy flow,
  • recovery patterns,
  • or coordinated multiscale activity.

APS therefore distinguishes:

DiagnosisBiosignatures
Direct evaluationIndirect inference
PerturbationalObservational
Tests organisationInfers organisation
Stronger evidenceMore uncertain

Biosignatures do not define life. They provide evidence that organised, self-maintaining activity may be present.

Key Point: APS diagnosis evaluates biological organisation directly where possible; biosignatures infer it indirectly from observable patterns of organised persistence.